IDENTIFIERS

dbEST Id:       8187852
EST name:       EST39
GenBank Acc:    BG467435
GenBank gi:     14269865

CLONE INFO
Clone Id:       (5')
Insert length:  516
DNA type:       cDNA

PRIMERS
Sequencing:     M13 reverse primer = caggaaacagctatgacc
PolyA Tail:     no

SEQUENCE
                GTTGTTTTTCTACTTCCTGTCCCCAGTTCCCACTGTGATTGCCCGTCGATTGTCCGGCAG
                TCTGGACTCCTCCAGCAGTGCCCTCATTGAGCTGTGTATTTTCCTTACTGTGGGAATCGT
                CATTTCTGCGATAGGATTACCAGTTGTCTTGGCCAGAACCTCTGTGATCCAGTGGGGAGC
                CTGTGGACTGGTTCTCGCAGGAAACGTGGTCGTCTTTCTGACAATCCTGGGATATTTCTT
                CGTGTTCGGCAATGAAGACCTAGACTACTCCTCATGGTGATAAACTCGGATCTGATTTCT
                TTTCAGTAATAAACGTAGACAATGTGACCCATTAATGTGTGCATGATTGCAACGGTTAGG
                TGCTGTGAGAATGATTGTTGACAGTAGGCGTGAGATTTAACCAGTGTATTTTCTCTTACA
                GACTGGCTCCCAGTGTAAGCATACTGCTTGTCATTTGTTACTTATAGTAAGATAAACACC
                AATCTATGTAGATATTATTTCCTATGATATATTTTC

Entry Created:  May 31 2001
Last Updated:   Aug 20 2003

PUTATIVE ID     Assigned by submitter
                Leptin receptor

LIBRARY
dbEST lib id:   8758
Lib Name:       Pacific oyster Lambda Zap Express gill cDNA library
Organism:       Crassostrea gigas
Tissue type:    Gill
Develop. stage: Adult
Vector:         Lambda ZAP Express
R. Site 1:      EcoRI
R. Site 2:      XhoI
Description:    A Pacific oyster gill cDNA library was constructed using the
                Lambda Zap Express/Gigapack cloning kit (Stratagene cloning
                systems). cDNA synthesis was carried out using an oligo-(dT)
                primer for the reverse transcription of 5_g of mRNA and the
                library was constructed by directional cloning EcoRI-XhoI
                based on manufacturers instructions. An insert:vector
                ligation ratio of 1:5 was chosen as most optimum. The lambda
                library was packaged with Gigapack III gold packaging
                extracts and plated on the E.coli cell line XL1-Blue MRF'.

SUBMITTER
Name:           Rafferty, G.P.
Lab:            Department of Microbiology
Institution:    National University of Ireland, Galway
Address:        Galway, Ireland
Tel:            00353 91 524411 2254
Fax:            00353 91 525700
E-mail:         gerard.rafferty@nuigalway.ie

CITATIONS
Title:          Identification of genes expressed in the gill tissue of the
                Pacific oyster (Crassostrea gigas) using expressed-sequence
                tags
Authors:        Rafferty,G.P., Powell,R.
Citation:       J Molluscan Stud 68 (4): 397-399 2002


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